Journal: Nature Communications
Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program
doi: 10.1038/s41467-025-68143-z
Figure Lengend Snippet: a Schematic of the coinjection model of D10 LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells with A549 cells in nude mice. b , c Fluorescence images of GFP-expressing A549 cells injected alone or coinjected with LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells (NIR790-stained) at D10 of OIS ( n = 6 per group); images were acquired on days 5, 15, and 28. One representative image is shown ( b ). The relative fluorescence intensity was quantified ( c ). d Tumors were excised after 28 days. e Tumor volumes were measured. f Immunoblot analysis of senescence markers in xenograft tumor samples. n = 3 biologically independent samples were analyzed, and similar results were obtained across replicates. g , h Xenograft tumor samples were subjected to H&E, SA-β-Gal, IL6, IGFBP2, CCND1, and STAG2 staining. Scale bars, 100 μm. Representative images ( g ); quantitative analysis of mean IHC staining ( h ). The data are presented as mean ± SD from six biological replicates with similar results. p values were calculated using a two-tailed unpaired Student’s t -test ( c , e , h ). The source data for the graphs in ( c , e , h ) and immunoblots in ( f ) are provided in the File.
Article Snippet: After the experimental endpoint, mice were euthanized, and tumors were excised, fixed, and subjected to sectioning and staining procedures, including H&E staining, SA-β-Gal staining, and immunostaining with antibodies for IL-6 (Proteintech, #21865-1-AP, 1:100), IGFBP2 (Proteintech, #11065-3-AP, 1:100), CCND1 (Proteintech, # 26939-1-AP, 1:1000), and STAG2 (Proteintech, #19837-1-AP, 1:100).
Techniques: Fluorescence, Expressing, Injection, Staining, Western Blot, Immunohistochemistry, Two Tailed Test
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
